Wet heat steam sterilizer is sterilized by hot steam.At the same temperature,the reason why the heat of dampness is better than that of dry heat is
1,Hot steam has a more damaging effect on cellular components.The presence of water molecules helps to destroy hydrogen bonds and other interacting weak bonds that maintain the three dimensional structure of the protein,making it easier to denature proteins.The water content of protein is inversely proportional to its solidification temperature.
2,Hot steam is more penetrating than hot air,which can kill microorganisms more effectively.
3.The steam has latent heat.When the gas is converted into a liquid,a large amount of heat can be released,so that the temperature of the sterilized object can be quickly increased.
Most vegetative cells of bacteria and fungi can be killed after treatment at 60 °C for 15 min.The cells of yeast and fungi should be heat resistant.It must be treated with a temperature above 80 °C to kill,and the spores of bacteria are more resistant to heat.It usually takes 15 minutes to kill at 120 °C.
Common methods for wet heat steam sterilizers are
atmospheric steam sterilizers and high pressure steam sterilizers.
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